| Author |
Message |
|
|
 |
Sun Aug 30, 2009 3:45 pm  |
You may want to read the information about these studies concerning Aloe Vera and AGEs:
Is it feasible for Aloe Vera to cause AGEs?
It is not only feasible, it certainly does happen. This is a classic example of the old adage nothing is all good or all bad. What it shows it that unless you have a wound healing problem, aloe vera is not a good thing to be using, and that it should only be used during the wound healing process. In fact, the wound healing benefit of aloe vera may well be an effect of the additional crosslinking of the collagen which makes the collagen network in the wound tougher.
Aloe vera seems to improves wound healing and inhibits inflammation, that is why so many products contain aloe vera. The major sugar found in aloe vera is mannose-6-phosphate (M-6-P), which as been investigated as the possible anti-inflammatory agent in aloe vera..
J Am Podiatr Med Assoc 1994 Feb;84(2):77-81
Anti-inflammatory and wound healing activity of a growth substance in Aloe vera.
Davis RH, Donato JJ, Hartman GM, Haas RC
Department of Biomedical Sciences, Pennsylvania College of Podiatric Medicine, Philadelphia.
Aloe vera improves wound healing and inhibits inflammation. Since mannose-6-phosphate is the major sugar in the Aloe gel, the authors examined the possibility of its being an active growth substance. Mice receiving 300 mg/kg of mannose-6-phosphate had improved wound healing over saline controls. This dose also had anti-inflammatory activity. The function of mannose-6-phosphate in A. vera is discussed.
Besides glucose, other reducing sugars, such as mannose, react with proteins to form advanced glycosylation end-products (AGEs), that are active in cross-linking collagen and elastin, the process that causes skin aging...
J Biol Chem 1992 Mar 15;267:5133-8
Immunochemical detection of advanced glycosylation end products in vivo.
Makita Z, Vlassara H, Cerami A, Bucala R
Laboratory of Medical Biochemistry, Rockefeller University, New York, New York 10021.
Reducing sugars react with protein amino groups to form a diverse group of protein-bound moieties with fluorescent and cross-linking properties. These compounds, called advanced glycosylation end products (AGEs), have been implicated in the structural and functional alterations of proteins that occur during aging and long-term diabetes. Although several AGEs have been identified on the basis of de novo synthesis and tissue isolation procedures, the measurement of AGE compounds in vivo has remained difficult.
As an approach to the study of AGE formation in vivo, we prepared polyclonal antiserum to an AGE epitope(s) which forms in vitro after incubation of glucose with ribonuclease (RNase). This antiserum proved suitable for the detection of AGEs which form in vivo. Both diabetic tissue and serum known to contain elevated levels of AGEs readily competed for antibody binding. Cross-reactivity studies revealed the presence of a common AGE epitope(s) which forms after the incubation of diverse proteins with glucose. Cross-reactive epitopes also formed with glucose 6-phosphate or fructose.
These data suggest that tissue AGEs which form in vivo appear to contain a common immunological epitope which cross-reacts with AGEs prepared in vitro, supporting the concept that immunologically similar AGE structures form from the incubation of sugars with different proteins (Horiuchi, S., Araki, N., and Morino, Y. (1991) J. Biol. Chem. 266, 7329-7332).
None of the known AGEs, such as 4-furanyl-2-furoyl-1H-imidazole, 1-alkyl-2-formyl-3,4-diglycosylpyrrole, pyrraline, carboxymethyllysine, or pentosidine, were found to compete for binding to anti-AGE antibody. These data further suggest that the dominant AGE epitope which forms from the reaction of glucose with proteins under native conditions is immunologically distinct from the structurally defined AGEs described to date. It has been found that phosphorylated reducing sugars are more active in glycating and cross-linking proteins than their parent molecules
Biochem Biophys Res Commun 1994 Aug 30;203(1):588-93 Advanced glycation of rat liver histone octamers: an in vitro study.
Gugliucci A Department of Anatomy, University of Montreal, Quebec, Canada.
The question whether histones could accumulate AGE products and whether this reaction could take place even on the native histone octamer, the basic nucleosome unit, was addressed in this study. In vitro AGE formation on rat liver native histones octamers by incubation with different sugars was assessed. We provide evidence for the in vitro formation of both pentosidine and total AGE fluorescence on histone octamers in a time and sugar concentration dependent fashion.
Ketoses (D-fructose and D-ribose) were more potent than aldoses. D-glucose-6-phosphate was three times as effective as D-glucose in generating AGE fluorescence. Advanced glycation of histone octamers led to the formation of cross links. For all sugars included in this study a similar pattern was observed: H2A and H3 bands disappear from electrophoretic runs.
Since aloe vera has mannose-6-phosphate as its major sugar, the M-6-P it contains would be active in the glycation of proteins and the formation of AGEs that cross-link proteins. In fact, aloe vera has been shown to increase the degree of cross-linking in collagen during wound healing..
Mol Cell Biochem 1998 Apr;181(1-2):71-6
Influence of Aloe vera on collagen characteristics in healing dermal wounds in rats.
Chithra P, Sajithlal GB, Chandrakasan G
Department of Biochemistry, Central Leather Research Institute, Adyar, Madras, India.
Wound healing is a fundamental response to tissue injury that results in restoration of tissue integrity. This end is achieved mainly by the synthesis of the connective tissue matrix. Collagen is the major protein of the extracellular matrix, and is the component which ultimately contributes to wound strength. In this work, we report the influence of Aloe vera on the collagen content and its characteristics in a healing wound.
It was observed that Aloe vera increased the collagen content of the granulation tissue as well as its degree of crosslinking as seen by increased aldehyde content and decreased acid solubility. The type I/type III collagen ratio of treated groups were lower than that of the untreated controls, indicating enhanced levels of type III collagen. Wounds were treated either by topical application or oral administration of Aloe vera to rats and both treatments were found to result in similar effects. |
|
|
|
|
|
|
|
| Wed Dec 04, 2024 8:35 pm |
If this is your first visit to the EDS Forums please take the time to register. Registration is required for you to post on the forums. Registration will also give you the ability to track messages of interest, send private messages to other users, participate in Gift Certificates draws and enjoy automatic discounts for shopping at our online store. Registration is free and takes just a few seconds to complete.
Click Here to join our community.
If you are already a registered member on the forums, please login to gain full access to the site. |
|
 |
|
|
