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Trizzy123
New Member
 
Joined: 20 Jan 2019
Posts: 7
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Fri Jun 21, 2019 8:42 am  |
Hi all. This is really a reply to my post a couple down on the main page where I talked about my latest DIY skincare routine. Sorry for the VERY delayed response to that. I had an ill parent and haven't had a moment of free time. Anyway, this post discuses my PRP methods, and later today I'll post on this thread the answers to the questions about my methylene blue and alpha arbutin recipes.
About the PRP (yes it is hardcore, and that's just the beginning of the crazy things I've done to my own face -like my own thread lifts). I've done this about 6 times so far, and definitely notice a difference. The biggest being an overall plumpness/firmness of the skin,the epidermis is smoother and evenness of pigment/skin tone. Overall I would say my skin looks younger and much healthier. And almost like it has better circulation- it just looks a lot more "alive".
Everything was purchased off EBay or Amazon. Here's what you need and some links, along with my process.
1. First I spread a thick layer of numbing cream over the entire area of the vein I am going to use. Then cover it with plastic wrap and let it "soak" for about 45 min. This numbs the area and you don't feel a thing when inserting the needle, allowing you to focus on the task and not the pain.
2. Once the area is good and numb, I sanitize with alcohol, open the butterfly needle package and tie the tourniquet. Wearing rubber gloves, I then insert the needle (the initial poke is at about 15 degrees, but then you level it off almost parallel with the skin to insert it the rest of the way. Veins are very close to the surface). There is instruction for this on YouTube. No blood will come out until you put the vacutainer tube on, so don't worry about blood gushing everywhere.
3. Pop on the vacutainer tube (it MUST have the ACD preservative in it. Can't use use just plain empty tubes). The tube will automatically stop filling one it's full. Then continue to change them out until I have 6 full tubes.
4. Remove the needle and apply bandaid.
5. I then take the tubes and place them in the centrifuge. Tubes must be balanced, so if you're doing 4 tubes, 2 on each side opposite each other. There is a study I used to determine the spin settings. It also has some good instructions on the whole process. First spin is at 1200rpm for 10 min.
6. After the first spin, you will see there are three layers. The top layer which is clear yellow liquid (platelet poor plasma), a small middle layer which is yellow and cloudy (platelet rich plasma or the "Buffy coat") and the bottom layer that is dark red (red blood cells). You want to extract the top and middle yellow layers (I use a long eyedropper) and leave the red blood cells. The yellow stuff is what you want, the red blood cells can be discarded. You'll get a little of the red blood cells when you suck it up in the eyedropper, but that's fine. So I extract the top and middle yellow layers from all 6 tubes and transfer all this to spin tubes. After the reduction I'm down to 2 full spin tubes of yellow liquid.
7. I then do my second spin at 2000rpm for 10 min. This further separates the clear yellow from the cloudy yellow. The CLOUDY layer is what you want. So I use my eyedropper to draw off the clear yellow layer (and discard) until I get down to the cloudy layer. I combine the cloudy layer from both tubes into one.
8. I then seterilize my face and microneedle all over. Once the microneedle has made tiny penetrations in the epidermis, I apply the PRP (the yellow cloudy liquid you are left with). I just take the eyedropper and drip it on my face a drop at a time and gently rub it in. Side note: studies say that the microneedle channels stay open for about 30 min before closing up, so you want to apply the PRP within 30 min of microneedling.
9. I leave all of the PRP on my face and let it dry (It's kind of sticky at first and then dries and kinda feels how dried egg yolks feel on your face). Then I apply aquaphor on top of it to serve as an occlusive and "seal in" the PRP. I sleep overnight with this on and wash it off in the morning.
I've done these treatments about every 2 weeks. It wasn't until my second or third treatment until I noticed a big difference. Not that the number of treatments mattered, but that it took a week or two for the first treatment to "kick in" and for the PRP to do its regeneration. But obviously multiple treatments are better.
The PRP can be left at room temp for up to 5 hours with no loss of effectiveness. If you don't use all of the PRP, it can be stored in the tube with the cap on in the fridge for up to 5 days and remain active. Each day of storage there are slightly less white blood cells, so ideally you'd use it right away.
I've also taken the pure PRP and transferred it to an insulin syringe and injected it into some old acne scars. I inject about .5 ml right into the center of the scar, with the needle depth being about 1 cm. Those have definitely improved by this treatment! The texture of the scar blends better with my skin now, but the biggest thing is the discoloration is pretty much gone. I had some that were hyper (dark) and some that were hypo (white or lacking any pigment). They all now have the same color as the surrounding skin. So the PRP definitely does something to correct the damage to the melanocytes.
Let me know if there is anything I've forgotten. Overall this treatment is a little time consuming- from the start of numbing my arm to the end where I'm applying the aquaphor takes about 2-2.5 hours. But it's not difficult or painful, and the results are WORTH IT. Oh, and you're saving yourself about $1000 per treatment doing it at home vs having a clinic do it!
Ok, so it won't let me post links to all the supplies I bought, but if you'd like to know just private message me and I'll send them to you! Along with the study I mentioned above. |
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Trizzy123
New Member
Joined: 20 Jan 2019
Posts: 7
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Fri Jun 21, 2019 8:55 am |
P.S. my latest "experiment" has been doing my own lipodissolve into my chin/jowls. It's a piece of cake to do, cheap (about $70 for 10 treatments)and really DOES work (way more than I expected), but there's a few tricks you need to know.
Let me know if anyone's interested in these instructions as well. |
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Fri Jun 21, 2019 1:31 pm |
Hi Trizzy,
Would love to hear more on your lipodissolve treatment 
Jackie xx |
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Sat Jun 22, 2019 9:31 am |
| Trizzy, how deeply do you microneedle for this procedure? |
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Mon Jun 24, 2019 1:08 pm |
| Wow Trizzy, I’m impressed. I don’t know how to send a PM, but I’d love to know your supplies for PRP. I am currently paying big $ for Vivace and just had my first treatment 5 days ago. I’d also love to hear more about your jowl and chin treatment. TIA |
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havana8
Moderator
Joined: 09 Sep 2005
Posts: 3451
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Tue Jun 25, 2019 4:28 am |
| Trizzy123 wrote: |
| Ok, so it won't let me post links to all the supplies I bought, but if you'd like to know just private message me and I'll send them to you! Along with the study I mentioned above. |
Just an FYI that when you reach 10 posts, you will be able to post links freely. |
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Tue Jun 25, 2019 9:16 am |
Hi Trizzy,
Thanks for posting this, I have been thinking of doing this myself and I'd love it if you could PM me with where you got your supplies!
Thanks! |
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Tue Jun 25, 2019 12:33 pm |
| I'd also like the details of where you get your supplies. Thank you for detailed post. |
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havana8
Moderator
Joined: 09 Sep 2005
Posts: 3451
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Wed Jun 26, 2019 9:10 pm |
| Following so I'll remember to look at this later. Sounds amazing! |
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GthGrl13
New Member
Joined: 29 Aug 2019
Posts: 6
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Sun Sep 01, 2019 11:13 am |
Trizzy where have you been all my life? Haha.You're a few steps ahead of me. Seriously impressed! I usually do tons of research on my own before any DIY, was just starting to research both of these things - so grateful for your detailed post!!! I'm definitely going to follow your instructions & do this. Already learning how to draw blood.
I'm also starting to look into aqualyx. Is that what you're using or something else? |
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